ASTM E1263-1997(2003) 哺乳动物骨髓红血球中微细胞核检验的实施

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【英文标准名称】:StandardGuideforConductofMicronucleusAssaysinMammalianBoneMarrowErythrocytes
【原文标准名称】:哺乳动物骨髓红血球中微细胞核检验的实施
【标准号】:ASTME1263-1997(2003)
【标准状态】:现行
【国别】:
【发布日期】:1997
【实施或试行日期】:
【发布单位】:美国材料与试验协会(ASTM)
【起草单位】:F04.16
【标准类型】:(Guide)
【标准水平】:()
【中文主题词】:原子;哺乳纲;骨髓;红细胞;分析
【英文主题词】:
【摘要】:Thisguideprovidesguidelinesfortheselectionofanimalspecies,dosageandsamplingconditions,samplingandscoringmethods,statisticaldesign,andanalysisofgenotoxicityassaysinwhichtheendpointmeasuredisthefrequencyofmicronucleatederythrocytesinmammalianbonemarrow.1.1Thisguideprovidesrecommendedguidelinesforperformingthemammalianinvivobonemarrowmicronucleusassay.Underappropriatetestconditions,measurementofthefrequencyofnewlyformedmicronucleatederythrocytesinbonemarrowprovidesaconvenientindexofchromosomaldamageinnucleatederythrocyteprecursorcells.Therationalefortheoccurrenceofmicronucleiinconjunctionwithchromosomaldamagehasbeendescribedpreviously(1).Thisguidedescribesconditionsunderwhichthefrequencyofmicronucleatederythrocytesinmammalianbonemarrowisanappropriatemeasureofinvivochromosomaldamage,andprovidesguidelinesforthedesignandtechnicalexecutionofassaysemployingthisendpoint.1.2Thefollowingguidelinesformammalianbonemarrowerythrocytemicronucleusassayshavebeenpublishedbyorganizationsconcernedwiththeevaluationofgenotoxicitytestdata.Thesereferencesshouldbeconsultedforrecommendationsondetailsnotcoveredindepthbythisguideandforrequirementsofspecificorganizationsorgovernmentagencies(2-6).1.3Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:B41
【国际标准分类号】:11_220
【页数】:7P.;A4
【正文语种】: